低氧对NG108－15细胞生长及分化的影响

EFFECT OF HYPOXIA ON TEE GROWTH AND DIFFERENTIATION OF NG108-15 CELLS

神经母细胞瘤和神经胶质瘤细胞融合的克隆细胞系ＮＧ１０８－１５细胞在含分化剂双丁基环化单磷酸腺苷（ｄＢｆｃＡＭＰ）的培养液培养后分化，成为具神经细胞特征的细胞。本实验利用四唑盐（ＭＴＴ）微量比色法，并结合焦油紫染色，测定和观察细胞的生长及分化状况，研究了低氧（２％Ｏ２＋９３％Ｎ２＋５／ＣＯ２）对未分化的，分化中的和已分化完成的ＮＧ１０８－１５细胞的影响。获得的主要结果是；低氧明显降低未分化细胞增殖和存活率，使分化完成的细胞大量死亡；低氧影响ＮＧ１０８－１５细胞的分化，使细胞在分化中出现体积膨大，突起短等异常特征，经焦油紫染色，胞质中无尼氏体（即不着色）的细胞增多。低氧是否可能使未分化ＮＧ１０８－１５细胞向更多地表达胶质细胞特征的方向分化？将是一个十分有趣的问题。

The neuroblastoma x glioma hybrid clone NG108-15 which were derived by fusion of mouse neuroblastoma clone N18TG6 with rat glioma clone C6BU1 proliferates in the presence of fetal calf serum, but differentiates when intracellular cAMP concentration is made to increase by addition of dBcAMP to the cultural medium.After differentiation, the cells express many neural properties. In this experiment,by means of anautomatic colorimetric microassay for quantifying the growth and Brilliant Cresyl Violett stain for revealing Nissl substance, the effect of hypoxia(2% O2) on the hybridcells was investigated. The results obtained were as follows:(1)Hypoxia depressed pramliferation of non-differentiated cells and induced death of differentiated ones.(2)Hypoxia inhibited differentiation, either non-differentiated or differentiating cells, in thedirection to be a neuro-like cell:some cells became large flat with short processes andhad no Nissl substance.Whether hypoxia would enable non-differentiated NG108-15cells to differentiate in a direction to express more properties of glial cell is an interestlug problem