低浓度氢清除法测定大鼠局部脑血流量

MEASUREMENT OF rCBF BY HYDROOEN GAS CLEARANCE WITH LOWER H_2 CONCENTRATION IN RATS

利用氢清除法测量动物局部脑血流量（ｒＣＢＦ）已被广泛应用于研究中。本文采用低于爆炸范围下限的３％Ｈ２浓度，它可以与任何比例的氮氧混合气体安全混合，且可避免吸纯氢期间的短暂缺氧，更为严格地控制了实验条件，增加了安全和准确性。本工作对吸１００％Ｈ２和吸３％Ｈ２以及吸３％Ｈ２１—１．５ｍｉｎ和吸３％Ｈ２５ｍｉｎ测得脑血流量分别作了比较，结果表明短期低浓度氢清除法能较好地反映动物的局部脑血流量。

H2 clearance method in measuring regional cerebral blood flow (rCBF) on animalshas been widely used in experimental research. It was found that 3 % H2 which is lowerthan the lower concentration limit guarded against occurrence of explosion could bemixed with any concentration ratio of N2 to O2 without causing transient hypoxia as often found by inhaling 100% H2 and thus made clearance measurement more accurateand safe. Comparison studies between inhaling 100% H2 and 3% H2 as well as inhaling 3% H2 for 1-1. 5 min and % H2 for 5 min were undertaken. Repeated measurements of 3 % H2 clearance for 1.5 min further showed that clearance test using low H2concentration for short term periods reflected better the rCBF of experimental animals.