山蝠和黑斑蛙乳酸脱氨酶同工酶及血糖浓度的季节变化

SEASONAL CHANGES OF LACTATE DEHYDROGENASE ISOZYMES AND BLOOD GLUCOSE CONCENTRATION IN NYCTUS NOCTULA AND RANA NIGROMACULATA

本工作采用聚丙烯酸胺凝胶电泳等方法，对山蝠（Ｎｙｃｔｕｓｎｏｃｔｕｌａ）和黑斑蛙（Ｒａｎａｎｉｇｒｏｍａｃｕｌａｔａ）在冬眠或蛰伏期与活动期的血清、脑、骨胳肌中的乳酸脱氢酶（ｌａｃｔａｔｅｄｅｈｙｄｒｏｇｅｎａｓｅ，ＬＤＨ）同工酶和血糖浓度进行了比较研究。ＬＤＨ同工酶及血糖浓度表现出明显的季节变化。与活动期的动物相比，在冬眠或蛰伏期中的动物的ＬＤＨ１缺乏、ＬＤＨ５明显增多（Ｐ＜０．００１），血糖浓度明显升高（Ｐ＜０．００１）。此外，ＬＤＨ同工酶和血糖浓度的季节变化在冬眠动物和蛰伏动物之间还存在着明显的差异。这些结果提示，ＬＤＨ同工酶含量和血糖浓度的季节变化不仅与动物的能量代谢水平相适应，而且显示了冬眠和蛰伏两种生理过程中的可能差异。

The blood sugar concentration and the lactate dehydrogenase (LDH) isozymes inserum, brain and skeletal muscle of Nyctus noctula during hibernation and of Rana Nigromaculata during dormancy and activity period were compared by using method ofpolyacrylanide gel electrophoresis. Both LDH isozymes and blood glucose concentrationshowed obvious seasonal changes. In contrast to animals during activity period, animals in hibernation and dormancy period lacked LDH1, while LDH5 and blood glucoseconcentration increased significantly (P<0.001).Also seasonal variations of LDHisozymes and blood glucose concentration were found to be different between animalgroup in hibernation and animal group in dormancy. The above results indicated thatseasonal changes of LDH isozymes and blood glucose concentration not only adapted tothe level of energy metablism but also showed difference between physiological statusduring hibernation and during dormancy.