鸡视网膜甘氨酸受体、乙酰胆碱受体及离子通道在两栖类卵母细胞中的表达和初步研究

STUDIES ON GLYCINE RECEPTOR,ACETYLCHOLINE RECEPTOR AND ION CHANNELS EXPRESSED IN AMPHIBIAN OOCYTES AFTER INJECTION OF CHICKEN RETINA mRNA

利用两栖类卵母细胞表达鸡视网膜ｍＲＮＡ，借助电压箝方法研究鸡视网膜中的神经递质受体和离子通道、结果表明，鸡视网膜中存在甘氨酸受体和Ｎ型的乙酰胆碱受体。但天冬氨酸和５－ＨＴ、多巴胺未能诱导电流反应。此外还检测到电压依赖性的离子流，主要为延迟整流型的外向钾电流和快速的内向钠电流。

Glycine receptor, acetylcholine receptor and voltage dependent ion channels expressed in amphibian oocytes after injection of chicken retinal mRNA were studied by using voltage clamp technique. The current response to glycine was proved as small,and the acetylcholine receptor was identified as N type. Aspartate, serotonin and dopamine induced no responses. In addition, voltage dependent ion channels were detected, and were identified as delayed rectifier potassium channel and sodium channel.