摘要
实验在幼年大鼠DRG标本上进行。应用细胞内记录观察了SP对GABA反应的调制作用。结果证明:(1)单独滴加SP(5×10(-6)-4×10(-5)mol/L)或浴槽灌流SP(10(-6)-5×10(-6)mol/L)不引起膜电位的改变或仅有轻微的去极化,但却能使GABA引起的去极化反应减小50.8±20.2%(±SD)(20/30);(2)单独滴加SP可使多数受检细胞APD50延长28.7±9.1%(±SD)(10/18);(3)在预加SP后,能使baclofen所引起的APD50缩短效应(20.6±2.9%,±SD)完全消除(4/12)或翻转成APD(50)延长19.3±8.9%(±SD)(8/12);(4)预加GABAB受体激动剂baclofen(10(-4)-10(-3)mol/L)30—90s后明显地抑制muscimol(10-4-10-3mol/L)引起的去极化反应,其抑制效应达54.4±18.8%(±SD)(17/20)。由于DRG神经元的胞体通常可用来作为研究初级传入终末的模型,因而本文实验结果提示:介导伤害性刺激信息的P物质在背角的释放,可能作用于初级传入终末,从而产生对抗GABA介导的突触?
Intracellular recordings were performed on the neurons of young rat DRG to study the modulatory effects of SP on the responses mediated by GABAA and GABAB receptors. In the majority of the neurons (20/30) the GABA induced depolarization was suppressed to 50. 8 ± 20. 2% by preapplication of SP (5×10-6-4×10-5 mol/L),which applied alone had no effect or only caused a slight depolarization.In addition,SP could increase APD50 by 28. 7 ± 9. 1 % in many neurons (10/18), and the baclofen-induced shortening of 20. 6 ± 2. 9 % in APD50 could abolished (4/12) or even reverse to a lengthening of 19. 3 ±8. 9 % in APD50 (8/12) by the preapplication of SP. Moreover, it was found that the activation of GABAB receptors could inhibit the succeeding response mediated by GABAA receptors. Since the soma of . the DRG neuron is generally considered as an accessible model for the primary afferent terminals, the results suggest that SP which is released in the dorsal horn during nociceptive stimulation can antagonize the responses mediated by GABAA and GABAB receptors.
出处
《生理学报》
CAS
CSCD
北大核心
1994年第5期441-450,共10页
Acta Physiologica Sinica
基金
国家自然科学基金
关键词
P物质
氨基丁酸
背根神经节
神经元
substance P
γ-aminobutyric acid
dorsal root ganglion
intracellular recordings
action potential duration