白细胞介素对大鼠海马培养神经元膜电特性的影响

EFFECTS OF INTERLEUKIN-1 AND INTERLEUKIN-2 ON ELECTROPHYSIOLOGICAL CHARACTERISTICS OF RAT HIPPOCAMPAL NEURONS IN CULTURE

用细胞内微电极记录方法研究了重组人白细胞介素－１β（ｒｈＩＬ－１β）和重组人白细胞介素－２（ｒｈｉＬ－２）对分散培养的新生大鼠海马神经元膜电性质的影响。采用压力脉冲微量给药技术将白介素施加于所记录的细胞表面。结果发现：１００Ｕ／ｍｌ浓度的ｒｈＩＬ－１β使受作用的海马神经元超极化４．２０±１．８６ｍＶ；１００Ｕ／ｍｌ浓度的ｒｈＩＬ－２使５０％受作用的海马神经元去极化１１．１２±３．７１ｍＶ，并伴有强烈的自发放电反应，而１０００Ｕ／ｍｌ浓度的ｒｈＩＬ－２使１００％受作用的神经元超极化３．２５±０．６３ｍＶ，这些神经元的膜阻抗均无明显变化。本实验结果提示ｒｈＩＬ－１β和ｒｈＩＬ－２可显著影响体外培养海马神经元的膜兴奋性。

The effects of recombinant human interleukin-113(rhIL-1P)and interleukin-2(rhIL--2)on the cultured rat hippocampal neurons were investigated with intracellular recording and extracellular micropressure ejection techniques.Experimental results showed that all of the neurons tested with rhIL-16(100 U/ml)exhibited a 4.20 11.86 mV membrane hyperpolarization.50% of the neurons treated with rhIL-2(100 U/ml)showed a depolarization of 11.12±3.71 mV accompanied by bursting activity,while in a still higher concentration(1000 U/ml)a 3.25±0.63 mV hyperpolarization was observed.All the above effects of rhIL-1β and rhIL-2 on the hippocampal neurons were completely reversible.The membrane resistance of the neurons was not significantly affected by these cytokines.These results suggest that the effects of cytokines IL-1 and IL-2 on the excitability of hippocampal neurons may be a way by which the immune system exerts a regulatory action on hippocampal function.