牙鲆（Paralichthys　olivaceus）全雌种苗生产开发技术

Studies on the Practical Feminization in Chinese Flounder Paralichthys olivaceus

牙鲆（Ｐａｒａｌｉｃｈｔｈｙｓｏｌｉｖａｃｅｕｓ）全雌种苗生产开发技术王新成（中国科学院海洋研究所青岛２６６０７１）鱼类染色体组操作技术是目前生物技术在鱼类养殖研究最具发展前景和最活跃的方面之一。可以培育适宜养殖的单性鱼苗或多倍体鱼苗。使其生长快，缩...

The paper introduces initial results of experiments of seeding production of feminization in flounder. Sperm was diluted over 50 times with Ringer's solution and irradiated by ultraviolet rays of 3000reg/mm2. The artificial fertilization was done with eggs immediately. Some fertilized eggs were hatched at normal temperature. Other eggs were treated 30 minutes in 0℃ seawater after fertilized 3 minutes in order to be induced diploid by meiotic gynogenetics which is achived through retention of second polor body. Haploids (84. 21%)diploids (77. 27%)of fertilized eggs were confirmed by chromosome counts respectively. Most of embryos of haploid died before hatching only a few were hatched larvae but none of them survived. Some larvaes of gynogenetic diploid were hatched,but the hatching rate was lower than normal. Further mass production of all -female in flounder is described.