外循环气升式反应器培养新疆紫草细胞

Large-scale Culture of Arnebia euchroma (Royle) Johnston Cells in 5L Outloop Airlift Bioreactor

采用两步培养法进行新疆紫草细胞悬浮培养及5L外循环气升式反应器扩大培养,探讨了培养过程中细胞生长、紫草色素合成与培养液的电导率、可溶性糖含量变化之间的关系。第一步培养时细胞生长迅速,但也有一部分色素合成,电导率及可溶性糖含量迅速下降;第二步培养初期电导率也开始下降,但当色素合成达到高峰并有一部分外泌到培养基后,电导率又开始回升。可溶性糖消耗很快,到后期已测不出其存在。因此通过监测培养液中电导率及可溶性糖的变化情况,可以为新疆紫草细胞大规模培养与色素合成提供有用的参数指标。

A two-stage culture method was established to culture Arnebia euchroma (Royle) Johnston cells in suspension and 5L outloop airlift bioreactor. Relationship between cell growth, shikonin formation and changes of conductivity, soluble sugar in cultured medium were studied. At the first stage, cells grew very fast, with the formation of a small amount of shikonin derivatives, and conductivity and soluble sugar decreased sharply. After cultured the cells in the second stage, conductivity decreased at first, and then grew up after a large amount of shikonin derivatives formed and secreted into the medium. By minotoring the changes of conductivity and soluble sugar, cell growth and shikonin derivatives formation were estimated in situ during the large-scale culture of A. euchroma cells.