十三碳二元酸发酵过程菌体生长期动力学模型及其应用

The Kinetic Models of Cell Growth Phase in the Fermentation Process for Producing Undecane Dicarboxylic Acid and Their Applications

介绍了由十三碳烷烃生产十三碳二元酸的发酵过程,对其中的菌体生长期的代谢过程进行了分析。提出了以CO_2释放率判断菌体生长状况的方法,据此可确定进入产酸期的最佳时间,建立了菌体生长期底物消耗及菌体生长的动力学模型,对模型参数进行了回归估值,并对菌体生长期进行了拟合。结果表明,模型的计算值和实测值吻合得较好,平均相对偏差为2.4％。利用所建模型对菌体生长期进行多种操作条件下的模拟计算,结果表明,提高蔗糖浓度及初始菌体浓度均能显著地提高菌体生长期结束时的菌体浓度。

The fermentation process for producing undecane dicarboxylic acid from tridecane is introduced. The metabolic characters of cell growth phase in the process were analyzed. It was proposed that cell growth can be indentified by the carbon dioxide production rate (CPR). The best time for switching into production phase can be decided on-line with CPR. The kinetic models of cell growth phase were established and parameters of the models have been estimated by regression. The calculated curves fit the experimental date quite well. The average deviation between those over cell growth phase is 2. 4%. Based on the kinetic models, computer simulations for several technological conditions of cell growth phase were fulfilled. From the results of the computer simulations, it can be seen that cell concentration at the end of cell growth-phase could be increased markedly by increasing both sucrose concentration and initial cell concentration.