细胞循环-代谢模型在面包酵母耐贮存力控制中的应用

Application of the Metabolic and Cell Cyclic Model System in the Control of Storage Stability for 5. cerevisiae

基于作者早期提出的面包酵母细胞循环-代谢模型设计了3次最优控制实验,目的是使发酵终了时的带芽细胞分率FBC趋于极小值,以期提高酵母的耐贮存力。实验成功地得以实施,不仅FBC的终值接近理论上的极小值,而且样品的耐贮存力得到了明显提高。另一方面,发酵力分析结果也表明,面包酵母的质量控制将随产品种类的不同而异。如对速用型鲜酵母的生产,FBC的控制就不宜以极小化为目标。

Three experiments for storage stability control of baker'S yeast were designed and carried out in laboratory scale fermenters. The feeding strategies of the fed-batch cultivation were determined by optimal simulation of the metabolic and cyclic model system for 5. cerevisiae, which was proposed and well tested earlier, and coupled with and objective function in which the quality index of storage stability FBC (Fraction of Buddiing Cells) was included,since the enhanced quality of storage stability is eventually expected through minimizing of FBC at the harvesting time. The experiments were realized very successfully. Not only the optimized FBC value have approached the theoretical minimum, but also the storage stability was greatly improved after minimization of FBC.