碳源对固定化细胞生产柠檬酸的影响

Effect of the Carbon Sources on Citric Acid Production by Immobilized Aspergillus niger

柠檬酸是利用微生物代谢生产的一种极为重要的有机酸,广泛应用于食品、饮料、化工、冶金、印染等各个领域。在国外,近10年来,利用固定化细胞生产柠檬酸已获得较广泛的研究,国内也有学者指出,柠檬酸发酵的趋向是利用固定化细胞进行连续化生产。而国内这方面的研究报道很少。我们利用海藻酸钙凝胶包埋固定化黑曲霉细胞生产柠檬酸,探讨了碳源种类及其浓度对固定化细胞生产柠檬酸的影响。现将结果报道如下。

The cells of Aspergillus niger W1-2 were immobilized in calcium alginate gel for the production of citric acid. The effect of carbon sources (sucrose, glucose lactose) and their concentrations on citric acid production were investigated. The experimental results showed that the souroce was more suitable carbon source than glucose and lactose. Immobilized A. niger cells needed a lower initial sourse concentration (120g/ L) than free cells (140g/L) in order to obtain maximal yield of citric acid. Morever, the carbon sucrose concentration had more remarkable effect on citric acid production with immobilized cells than with free cells.