短梗霉多糖发酵条件的研究

Studies on the Conditions of Fermentation of Pullulan by Aureobasidium pullulans

在摇瓶发酵条件研究的基础上。于16L自控发酵罐上进行了罐上发酵条件优化研究,发现以10％淀粉水解物为碳源时,淀粉水解物的最适DE值为40—50,发酵培养基中的硫酸铵最适用量不同于摇瓶发酵时的量,种龄和接种量、通气量、罐压、搅拌速度和搅拌叶轮挡数等均对多糖的产生有较大的影响。另外还进行了发酵过程的动力学的研究。

The studies on suitable conditions for pullulan fermentation according to the results obtained from the shaking flask tests was carried out in 16L auto-controlling fer-mentor. It fount that the optimal DE value of starch hydrolyzate was 40-50 when 10% starch was used as carbon source. The optimal concentration of ammonium sulfate in the medium for fermentor was different from that of the shaking flask. The fermentation kinetics and effects of seed age, seed volume, airflow rate, pressure of tank, agitation speed and number of vane group on the production of pullulan were investigated.