固定化光合细菌利用有机物产氢的研究

Research on Hydrogen Production from Organic Compounds with Immobilized Cells of Photosynthetic Bacteria (PSB)

应用固定化细胞技术包埋荚膜红假单胞菌(Rhodopseudomonas capsulata)菌株386,研究在光照下利用有机物产氢的特性。实验观察到,光照培养120小时,悬浮培养物的产氢量为68.2ml,比产氢速率为104.1ml H_2/g(生物量)·h;用琼脂包埋后,其产氢能力得到改善,产氧量和比产氢速率分别达到128.4ml和195.8ml H_2/g·h。该菌株除可利用苹果酸外,还可利用葡萄糖、乳酸、内酸等基质高效地产氢。基质浓度只有控制在适当水平时,才具有较高的基质转化产氢效率。此外,菌体生物量、菌龄、培养液pH、光照强度、光照/黑暗时间比以及温度对产氢过程均有不同程度的影响。

The characteristics of hydrogen photoproduction from organic compounds by immobilized Rhodopseudomonas capsulata 386.were studied in batch culture. On the condition that free cells hydrogen-producing cultures were cultured in light for 120 hours, hydrogen production and specific H2 production rate were 68.2ml and 104. 1ml H2/g ?h, respectively. The activity was largely increased when immobilized cells by agar gels was used with hydrogen production up to 128. 4ml and specific hydrogen production rate up to 195. 8ml H2/g ?h. It was proved that in addition to malate. this bacterium could also used glucose, lactate and propionate as hydrogen production substrate and only at appropriate substrate concentration it reached a higher conversion effciency of substrate to H2. Age of inoculum, pH value, light intensity, light dark time and temperature all have different effect patrns.