虫光素酶N端16个氨基酸与催化活性密切相关

The First Sixteen N-teriminal Amino Acids ofFirefly Luciferase involve in Catalytic Activi-ty.

把全长虫光素酶基因及其缺失突变体（５’端缺失４８个核苷酸）分别克隆到分泌型表达载体ｐＩＮ－Ⅲ－ｏｍｐＡ３，前者转化体能表达高活性虫光素酶，而后者完全丧失酶活。该结果表明虫光素酶Ｎ端１６个氨基酸与酶活性密切相关。

he full-length intronless firefly luciferasegene and its deletion mutant which lacked 48nucleotides from the initiation codon (ATG)were inserted into the high expression secre-tion vector. pIN-Ⅲ-ompA3. and introducedinto E. coli cells in which high level and no lu-ciferase activity were detected. respectively.This result shows that the first sixteen N-ter-minal amino acids of firefly luciferase involvein catalytic activity.