摘要
首次报道了用乙醇作梯度洗脱有机相分离PTC-氨基酸的新方法。同乙腈相比,乙醇毒害性小、价廉、易得。在优化的色谱条件下,乙醇洗脱分辨率、灵敏度、准确度均佳,可成为PTC-氨基酸分析的一种极有前途的新方法。
Analysis of phenylt 2hiocarbamyl (PTC) aminoacids with ethanol as organic eluent is de-scribed. Compared with acetonitrile elutionsystem, ethanol is less toxic, easier to obtainand much cheaper. Under optimized chro-matographic conditions, the resolution, sensi-tivity and accuracy are excellent.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第2期162-165,共4页
Progress In Biochemistry and Biophysics