植物叶片的相位解析光声光谱和光声相位谱

Phase-Resolved Pbotoacoustic SPectroscopyand Photoacoustic Phase Spectrum of the In-tact Leai.

用相位解析法获得的未损伤植物叶片叶肉组织的光声光谱，与叶片的光声相位谱进行了比较．发现植物叶片的光声相位谱与叶绿体光吸收带之间呈现互补关系．叶片表皮的光声相位谱存在持有的波谷结构．不同调制频率的光声相位谱有较大的差异．这些现象表明光声相位谱与光声光谱一样，也可对未损伤的层状生物样品作深度剖面分析．

he phase-resolved photoacoustic spectroscopyhas been used to analyse the pigmental distri-bution in intact plant leaves and has been com-pared with the photoacoustic phase spectrumof the leaves. The inverse correlation betweenthe photoacoustic phase spectrum and absorp-tion bands of chloroplasts has been observed.The characteristic valley exists in the photoa-coustic phase spectrum of the leaf cuticle. Inaddition, there are some differences in thephotoacoustic phase spectra obtained at differ-ent modulation frequency. The phenomenashow that photoacoustic phase spectra can alsobe used for the nondestructive depth-profileanalyses of biological sample as good as pho-toacoustic spectroscopy.