目前最高分辨率的电泳──固相pH梯度等电聚焦

A Current BreakThrough ElectropboresisTechnique with Superinost Resolution: Immo-bilized PH Gradients Isoeltctrofocusing.

固相ｐＨ梯度等电聚焦是国际上８０年代的新型电泳技术。利用一系列具有弱酸和弱碱性质的丙烯酰胺衍生物滴定时，在滴定终点附近形成的ｐＨ梯度并参与丙烯酰胺的共价聚合，从而形成固定的不随环境电场等条件变化的ｐＨ梯度，该方法具有比传统载体两性电解质等电聚焦更高的分辨率、更大的上样量。可用于分析和制备相近ｐＩ的蛋白质，多肽等。

The mentioned immobilized PH gradients iso-electrofocusing is a electrophoresis techniquedeveloped in 80' s. An approximate linear PHgradient is genarated by titrating weak acidicand basic acrviamide derivaties which then co-valent bound into the polyacrylamide matrix.The PH gradient is stable and independent ofelectric field fluctuation. The present methodprovides higher resolution and larger loadingcapacity comparing with conventional carrierampholyte isoeletrofocusing. It can analyseand purify protein with only minor PI differ-ence.