P53，Rb和c－myc基因在人脑原发性肿瘤中的转录表达

The Expression of p53. Rb and c-myc GenemRNA in Human Primary Brain Tuinor.

采用ＲＮＡ斑点杂交分析，对２１例人脑原发性胶质瘤和１１例人脑膜瘤中ｐ５３，Ｒｂ和ｃ－ｍｙｃ基因转录水平的表达进行研究。发现４８．４％的肿瘤中ｐ５３基因表达减弱，２１．９％的肿瘤中Ｒｂ基因表达减弱；７１．９％的肿瘤中ｃ－ｍｙｃ基因表达增强。在ｐ５３基因表达减弱的１５例病例中有１３例（８０％）ｃ－ｍｙｃ基因表达增强。结果表明，ｐ５３基因表达减弱和ｃ－ｍｙｃ基因表达增强与人脑原发性肿瘤的发生有关．

1 human primary brain gliomas and 11 humanmenigiomas were examined with RNA dot blothybridization for the expression of p53, Rband c-myc gene. It was found that the level ofp53 gene expression is lower in 48. 4% (15/31)of the tumors tested than that of normal braintissues ; the level of Rb gene expression is low-er in 21. 9% (7/32) for the tumors tested thanthat of normal tissues; and the level of c-mycgene expression is higher in 71. 9 % (23/32) forthe tumors tested than that of normal tissues.Interestingly, in 13 of the tumors tested, thelevel of p53 gene expression is lower and thelevel of c-myc gene expression is higher.These results suggested that the expressivedecrease of p53 gene and the expressive in-crease of c-myc gene are relative to the genera-tion of human primary brain tumor.