摘要
建立了以毛细管电泳为基础的测定蛋白激酶A活性的新方法,可作为激酶测活的通用方法.此法基于底物及其磷酸化产物很容易在毛细管电泳中分开,且酶活力可用积分值计算,同时又发展了连续进样技术,能在一次电泳中同时进行10个以上的酶活性测定,新方法操作简单,灵敏度和精确性均优于常规的同位素法.
new assay of protein kinase A using capil-lary electrophoresis has been established.It isa universal and useful method for kinase activi-ty. The method based on principle that sub-strate (kemptide)and product (phosphory-lated kemptide) are easily seperated by capil-lary electrophoresis and the enzyme activitycan be calculated on the integrated area. Acontinous sampling technique which can anal-yse more than 10 samples in one run has alsobeen developed.The new method is easy tooperate and its accuracy and sensitivity arehigher than that of conventional isotopicmethod.
出处
《生物化学与生物物理进展》
CSCD
北大核心
1994年第3期267-267,270,共2页
Progress In Biochemistry and Biophysics
基金
"863"计划资助
关键词
磷酸化
毛细管电泳
蛋白激酶
活性
protein kinase A activity.capil-lary electrophoresis
phosphorylation