一种新的测定蛋白激酶活性的方法──毛细管电泳测定蛋白激酶A的活性

A New Assay for Kinase Activity: The Deter-mination of Protein Kinase Using CapillaryElectrophoresis.

建立了以毛细管电泳为基础的测定蛋白激酶Ａ活性的新方法，可作为激酶测活的通用方法．此法基于底物及其磷酸化产物很容易在毛细管电泳中分开，且酶活力可用积分值计算，同时又发展了连续进样技术，能在一次电泳中同时进行１０个以上的酶活性测定，新方法操作简单，灵敏度和精确性均优于常规的同位素法．

new assay of protein kinase A using capil-lary electrophoresis has been established.It isa universal and useful method for kinase activi-ty. The method based on principle that sub-strate (kemptide)and product (phosphory-lated kemptide) are easily seperated by capil-lary electrophoresis and the enzyme activitycan be calculated on the integrated area. Acontinous sampling technique which can anal-yse more than 10 samples in one run has alsobeen developed.The new method is easy tooperate and its accuracy and sensitivity arehigher than that of conventional isotopicmethod.