摘要
应用免疫沉淀法测定了乳酸脱氢酶同工酶1(LD1).血清与抗血清用量为10:1,加入抗血清5min后再加入与血清量相等的饱和硫酸铵溶液.离心后测定上清液中的LD.总酶活性在618U/L内线性关系良好.二份标本批内精度CV值分别为3.76%和4.70%,批间CV值为7.00%,免疫沉淀法与电泳法高度相关(n=22,r=0.976).72例正常人LD参考值为102.31±16.4U/L,LD1为23.65±4.39U/L,LD1/LD为23.12±3.85%.免疫沉淀法的优点是特异性高,操作简单,可用于自动生化分析仪.精确度高,线性关系好,测定范围宽,是测定LD1的理想方法.
soenzyme 1 of lactate dehydrogenase (LD1)was measured by an immunoprecipitationmethod. The antibody to M subunit of LD wasadded to the patient’s serum and incubated for5 min,at room temperature. The ratio ofserum to antibody was 10:1.After incuba-tion,a saturated ammonium sulphate solutionwas added with the same volume as serum.Then.centrifuged to precipitate all M-contain-ing isoenzyme (LDZ LDS) as insoluble anti-gen-antibody complex. Determined the residu-al activity of LD in supernatant fluid. The re-lationship Between the LD activity and ab-sorbance was linear up to 618U/L.Within-runcoefficient of variation (CVs) of two sampleswere 3. 8% and 4. 7%. Day-to-day coefficientof variation(CVs)was 7.0%. The correlationof immunoprecipitation method and elec-trophoretic procedure were cinsistent (n=22,r=0. 976).Reference values for total LD andLD 1 were 102.3± 16.4U/L and 23.7±4.4U/L respectively.The ratio of LD1 to to-tal LD was 23.1±3. 9%. The advantages ofimmunoprecipitation method were:high speci-ficity.good precision and linearity, easy oper-ation.Immunoprecipitation method was verysuitable for measuring LD1 and could adapt-able to the automatic analyzer.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第3期262-264,共3页
Progress In Biochemistry and Biophysics
关键词
免疫沉淀法
乳酸脱氢酶
同功酶
isoenzyme 1 of lactate dehydroge-nase (LD1).immunoprecipitation method, an-ti-M antibody