极谱氧电极法测定超氧化物歧化酶活性的改进

Modifications on the Polarographic OxygenElectrode Method for Superoxide DismutaseActivity Determination.

对ＳＯＤ的极谱氧电极测定法做了如下修改：ａ．室温测定，ｂ．酶活性用标准ＳＯＤ标定，ｃ．反应在磷酸缓冲液中进行，ｄ．增大邻苯三酚的用量．改进后克服了易在电极薄膜表面产生气泡等问题，测定灵敏度及线性范围增大．

he following modifications to the polaro-graphic oxygen electrode method for SOD ac-tivity determination were made: (1) directlydetermining at room temperature, (2) intro-ducing standard SOD as activity unit standard,(3) using phosphate buffer as reaction medi-um. and (4) increasing the pyrogallic acidused. These modifications result in: (1) theabolishment of the bubbles easily produced onthe electrode surface which severely interferewith the determination, (2) increased sensitiv-ity of determination and (3) broadened linearrange of SOD activity.