电荷耦合器件荧光成象及生物学应用

Fluorescence Digital Imaging Using a Charge-Coupled Device Camera and Its Biological AP-plications.

冷却慢扫描电荷耦合器件照相机附在荧光显微镜上组成了一个系统，可把一系列成象进行贮存，通过标记颗粒的位置、强度以及强度分布的差别，在每幅象上可区分５００个荧光点。荧光点的运动可以跟踪，通过分析可区分不同的运动方式，在活细胞表面，已观察到受体有直接扩散，随机扩散和局部随机扩散三种运动方式。电荷耦合器件的高灵敏度使观察过程中只产生少量的漂白。现介绍这系统的测量原理和它在生物学上的应用。

A system, using a cooled slow-scan chargecoupled device camera attached to a fluores-cence microscope. allows a series of images tobe stored, up to 500 fluorescent spots (la-belling particles) per image can be identifiedby position and intensity. The movement ofthe spots can be traced. The tracking methodallows the mobility to be analysed. The recep-tors of influenza virus and low density lipopro-tein on fibroblast have three different types ofmotion: random motion, directed motion andmotion limited to a domain. The high sensitiv-ity of the systern leads to short exposures andlittle photobleaching during the observations.The measurement principle of this system andits biological applications were described.