以核多角体病毒为载体在家蚕中生产外源蛋白

Production of the Useful Protein in the Silk-worm Using the Bombyx mori Nuclear Polyhe-drosis Virus as a Expression Vector.

以家蚕核多角体病毒（ＢｍＮＰＶ）为载体，在家蚕幼虫或家蚕培养细胞系中表达的外源基因越来越多，其表达的产物已涉及到医用药物、医疗诊断、疫苗生产、生物防治等诸多领域，文章就ＢｍＮＰＶ的特性及其基因组构造，多角体蛋白基因的特性，重组ＢｍＮＰＶ的构建及其在家蚕幼虫体内和细胞系中的表达，ＢｍＮＰＶ－家蚕表达系统的外源蛋白生产效率及其应用等各个方面作了全面、系统的综述。

More and more foreign genes have been ex-pressed in the silkworm larvae or silkworm celllines using the Bombyx inori nuclear polyhe-drosis virus (BmNPV) as a expression vector.The expressed products involve in many fieldssuch as pharmaceutics. medical diagnosis, vac-cineperoduction and biological control. Thecharacteristics of BmNPV and its genomestructure. characteristics of polyhedrin gene.construction of recombinant BmNPV and itsexpression in the silkworm larvae and cell line,and efficiency of production for the foreigngene products expressed in the silkworm-Bm-NPV system and application of the expressedproduct were described systematically in thereview.