TGFα反义寡聚核苷酸对膀胱癌BIU 87细胞株的作用

The Inhibitiou of BIU87 Cell Proliferation andTGFα mRNA Expression by an TGFa Anti-sense Oligodeoxynucleotide.

报道了２３碱基组成的ＴＧＦα反义寡聚核苷酸和对照寡聚核苷酸对人膀胱移行细胞癌ＢＩＵ８７细胞增殖及其ＴＧＦαｍＲＮＡ表达的作用，结果表明：ＴＧＦα反义寡聚核苷酸抑制体外培养的ＢＩＵ８７细胞的增殖、ＤＮＡ合成和ＴＧＦαｍＲＮＡ的表达，进一步证明了ＴＧＦα在ＢＩＵ８７细胞恶性增殖中的重要作用．

Two 23-mer unmodified oligodeoxynucleotideshave been synthesized, one to hybridize withthe region across the transcription initiationsite of TGFa cDNA called antisense, and thesecond with an identical base composition, butthe nucleotide sequence randomized. The ran-dom oligodeoxynucleotide was used to controlfor any non-specific effects of theoligodeoxynucleotides. TGFα antisense inhib-ited cell proliferation and DNA synthesis from31 /00 to 44/00 and 25. 8/00 to 88. 0% respective-ly. This effect is specific and dose-responded.However. at the same condition, the inhibi-tion by random is only 10% to 17% and 17%to 27%. This effect is not dose-responded. ByRNA dot blot analysis , the addition of 3 μmol / LTGFα antisense and random upon BIU87 cellsresulted in 61. 4% and 42. 5% inhibition inTGFα mRNA expression respectively. Theseresults suggested that TGFα may play an im-portant role in the proliferation of BIU87 cellline and provide a rational basis for the devel-opment of selective cancer therapeutical ap-oroaches .