胎盘型谷胱甘肽S－转移酶基因在胃癌中的表达

GST-π Gene Expression in Gastric Tumor.

用Ｄｉｇ－ＧＳＴ－πｃＤＮＡ探针分子杂交方法，检测了正常胃组织、胃癌及相应癌旁正常组织中ＧＳＴ－πＤＮＡ和ＧＳＴ－πＲＮＡ水平．发现ＧＳＴ－πＤＮＡ水平没有明显变化，而ＣＳＴ－πＲＮＡ在８例胃癌组织中有６例高于正常胃组织，在１２例低分比腺癌中有７例癌旁正常组织高于相应癌组织．表明ＧＳＴ－π基因表达增加与胃癌有关，而且早于细胞形态的变化．

Increased expression of the glutathione S-transferase (GST: E, C, 2, 5, 1, 18) π classisoenzyme is associated with both tumor andpreneoplastic tissues. In order to further char-acterize the alteration of GST-π gene expres-sion during progression of carcinoma, boththe levels of GST-π DNA in one normal gas-tric tissue and 20 gastric tumor with perineo-plastic normal tissue. and the GST-π RNA inthe normal gastric tissue and 12 gastric poorlydifferiented adenoearcinomas with correspond-ing perineoplastic normal tissues were testedusing Dig-GST-π cDNA probe by Dot blot hy-bridization. No significant change of GST-πDNA level, but the expression level of GST-πRNA in 6 of eight gastric tumors was higherthan in normal gastric tissue, and in 7 peri-neoplastic normal tissue of twelve poorly dif-ferentiated adenocarcinoma was higher thanthat in its corresponding tumor. This suggeststhat the elevation of GST-π gene expression isrelated to gastric tumor, and earlierthan thechanges of cell morphology.