摘要
白细胞介素-2(IL-2)是重要的免疫调节因子,近来发现还有中枢镇痛作用,用不同IL-2突变体测定其对大鼠痛阈的影响,发现完全丧失免疫刺激作用的20Leu-IL-2(20Asp→Leu)仍能显著提高大鼠的痛阈,其作用强度与天然IL-2无显著差异,而另一突变体45Val-IL-2(45Tyr→Val)虽保留免疫学活性却不能提高大鼠的痛阈。这些结果证明IL-2分子中具有镇痛作用与具有免疫作用的功能位点是相互独立的;IL-2分子中第45位Tyr对IL-2镇痛作用的发挥起重要作用。
nterleukin-2(IL-2)is an important immuneregulator.Recently it is found that IL-2 is alsoan analgesic molecule.Using the potassiumiontophoresis as pain stimulus to induce tail-flick and taking the intensity of current (mA)at the moment of the response as the painthreshold (PT),it is reported that mutant 20Leu-IL-2 (20Asp-Leu),which could not bindto the βsubunit of IL-2 receptor and thus hadno immune activity, could increase the PT ofthe rats, indicating that the analgesic and theimmunal functions be related to two differentdomains in IL-2 molecule. Mutant 45Val-IL-2(45Tyr-Val),which had immunal activity,had no analgesic effet,suggesting that the45Tyr be crucial for the analgesic effect of IL-2and the analgesic domain be located around the45Tyr.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第6期518-520,共3页
Progress In Biochemistry and Biophysics
基金
863资助
关键词
白细胞介素2
定位突变
止痛
interleukin-2.site-ditected muta-genesis,analgesia,structure-function studies.central nervous system