摘要
本文利用SDS-聚丙烯酰胺凝胶电泳方法,定量研究了体外培养的软骨细胞和软骨组织基质中Ⅱ型胶原蛋白的含量。结果表明氧自由基(·O-2和·OH)和具有自由基性质的物质(黄腐酸,镰刀菌毒素)可使软骨细胞合成,分泌异常的非Ⅱ型的胶原蛋白,同时,硒化合物可明显地抑制此种效应。
In this paper, a method of determining the percentage of type Ⅱ collagen in extracellular matrix of cultured chondrocytes or caritilage tissue in terms of the spectrophotometric densities of specific cyangen bromide peptide bonds on SDS-polyacrylamide gels has been used. The results show that the free radical generating systems, including Fe(Ⅱ)-EDTA,Xanthine Oxidase-Xanthine. Fulvic acid and Fusarium toxin, can cause chondrocytes to synthesize and secrete mainly type Ⅰ collagen instead of the normal type Ⅱ collagen. In addition, The collagen type change can be inhibited by Se(Ⅳ) compounds.
基金
国家"八五"科技攻关课题