摘要
依据Ｔｒｉｎｉｃｋ－Ｅｐｐｅｎｂｅｒｇｅｒ对鸡骨骼肌Ｍ蛋白的提取方法，由人骨骼肌中得到的Ｍ蛋白粗提物除含分子量为１６５０００的Ｍ蛋白外，还含有分子量为１８５０００和１４００００（Ｃ成分）的两组分。由于在粗提物中未发现分子量为９００００的磷酸化酶，我们将最终纯化步骤中的亲和层析改为制备电泳，同样获得了纯化的Ｍ蛋白。
Three protein components were obtained from the crude extract of human skeletal muscle M-protein by Trinick-Eppenberger's method for preparation of M-protein from chicken skeletal muscle.There MW were 165000(M protein), 185000 and 145000 respectively. The MW 185000 may probably be myomesin, but whether the 140000 component is the degraded product of M-protein or some other protein is still not clear. The pure M-protein was obtained by the preparative electrophoresis instead of affinity chromatography.
基金
国家自然科学基金
