摘要
用紫外共振拉曼光谱研究了ＡＤＭ与小牛胸腺ＤＮＡ相互作用，分析表明：ＡＤＭ插入ＤＮＡ的ＧＣ－ＣＧ位置；ＡＤＭ与ＤＮＡ之间的主要相互作用是蒽环π电子与碱基Ｇ和Ｃ的π电子形成的π－π电子相互作用，并通过碱基Ｇ、Ｃ的ＮＨ＿２的氨的孤对Ｐ电子与ＡＤＭ的π电子形成的π－Ｐ电子相互作用以及ＡＤＭ和ＤＮＡ碱基Ｇ、Ｃ和ＰＯ＿２之间形成的氢键使相互作用加强；ＡＤＭ插入ＤＮＡ使其构象产生一定变化，但未破坏ＤＮＡ碱基对间的氢键。
The interaction of anticanaer-drug ADM (Adriamycin) with calf thymus DNA was studied by ultraviolet resonance Raman spectroscopy. The analysis demonstrated that ADM intercalates into GC-CG sites of DNA. The main interaction between ADM and DNA is the π-π electronic interaction between π-electron of anthracycline andπ-electron of guanine and cytosine . It is also reinforced via π-p electronic interaction between lone pair p-electron of nitrogen of NH_2 group of guanine or cytosine and π-electron of ADM chromophore and the hydrogen bonds of ADM with cytosine, guanine and PO_2. The conformation of DNA is changed when ADM intercalates into DNA, but it does not disrupt the hydrogen bonds between bases of DNA.
基金
国家教委重点基金
关键词
阿霉素
紫外共振拉曼
抗癌药
DNA
Anticancer-drug
Adriamycin
Ultraviolet resonance Raman
Interaction with DNA
