反义RNA对人胃癌细胞生长抑制及恶性表型的阻断作用

Inhibition of Growth Ability and Tumorigenicity of Human Gastric Carcinoma Cell Iine BGC-823 Expressing c-Ha-ras Antisense and Sense RNA

ｒａｓ原癌基因的点突变是人胃癌发生发展的重要机理之一。利用能表达ｃ－Ｈ－ｒａｓ癌基因反义ＲＮＡ的质粒，导入人胃癌细胞系ＢＧＣ－８２３，研究了ｒａｓ癌基因反义ＲＮＡ对人胃癌细胞生长及恶性表型的作用，结果表明，ｃ－Ｈ－ｒａｓ反义ＲＮＡ可引起ＢＧＣ－８２３生长速率及形态的变化，在半固体培养基中细胞集落形成能力减弱，部分地抑制了ＢＧＣ－８２３在裸鼠体内的致瘤性。ｃ－Ｈ－ｒａｓ反义ＲＮＡ对其ＲＮＡ的过量表达呈特异性抑制作用。

Our previous work showed that point mutation plays an important role in the activation of H-ras protooncogene. We have inserted first exon and its upstream 2. 0 kb fragment of c-Ha-ras into shuttle vector pZipneoSV (X). The constructs of pZipneo2.0S and pZipneo2. 0AS of H-ras expressed sense and antisense RNA, respectively. We have introduced the constructs of pZipneo S and AS which expressed c-Ha-ras sense and antisense RNA into BGC-823 cell line with retrovirus infection. The colonies of G418 resistant were analysed on morphology, growth ability in serum free medium or soft agar and tumorigenicity in nude mice. Our data demonstrated that the growth ability or colonies forming efficiency in soft agar and tumorigenicity in nude mice were inhibited by c-Ha-ras antisense RNA.