CCK－8和NDAP对大鼠脑和脊髓组织c－fos表达的影响

Effect of CCK-8 and NDAP on c-fos Expression in Rat Brain and Spinal Cord Tissues

为探讨八肽胆囊收缩素（ＣＣＫ－８）和阿片肽相互作用的分子机理，利用抗体免疫沉淀技术研究了ＣＣＫ－８与ＮＤＡＰ（κ阿片受体激动剂）对大鼠脑（去皮层和小脑）和脊髓背柱组织Ｆｏｓ蛋白的影响。结果表明，０．１μｍｏｌ／Ｌ ＣＣＫ－８可显著刺激脑和脊髓组织中Ｆｏｓ蛋白增加（分别是对照组的３．８倍和３．６倍）。相同浓度的ＮＤＡＰ对Ｆｏｓ蛋白的生成亦有一定的诱导作用，分别是对照组的２．７倍和２．６倍。

For elucidating the molecular mechanisms underlying the interaction of cholecystokinin octapeptide (CCK-8) and opioids, we investigated the effects and interaction of CCK8 and NDAP (a k opioid receptor agonist) on Fos protein expression in rat brain and spinal cord tissues by in vitro immunoprecipitation method. Under 0. 1 μmol/L CCK-8 stimulation,there were markedly increase of Fos protein levels both in brain (3. 8 fold increase) and in spinal cord(3. 6 fold increase). 0. 1μmol/L NDAP also exhibited inducing effect on Fos protein production, Fos protein level increased 2. 7 and 2. 6 fold respectively in brain and spinal tissues. The combined effect of CCK-8 and NADP on c-fos expression in rat brain was the same as the level induced by CCK-8 alone. However, in spinal cord the Fos protein level induced by CCK-8 combined with NDAP was much higher than that induced by CCK-8 alone.The results indicated that both CCK-8 and NDAP could induce c-fos expression directly, but the patterns of their combined effects on c-fos expression in brain and in spinal cord were different.