人和牛血清白蛋白的极谱测定

Polarographic Determination of Human and Bovine Serum Albumin

人或牛血清白蛋白溶液调至ｐＨ１０并在４℃左右放置３０ｈ，诱导出重复性良好的极谱峰。然后把试样溶液调至适当ｐＨ并记录极谱电流。在浓度低于１．０×１０－５ｍｏｌ／Ｌ时，峰电流对浓度作图是直线，试样浓度可从图上直接读出。对影响白蛋白测定的诸因素作了详细研究和讨论。结果对富含硫的蛋白质的极谱研究有参考价值。

After the human or bovine serum albumin solution was adjusted to pH 10 and stood for 30h at around 4℃, a reproduceable polarographic peak is induced. Then,the sample solutions were adjusted to suitable pH, and the peak currents were recorded. When the concentrations are lower than 1. 0×10-5mol/L, the plot of peak currents versus concentration is a straight line, so that sample solution concentration can be read directly from the graph. The factors of influence on the polarographic determination were investigated and discussed in detail. The results may throw a light on the polarographic study of sulphur rich proteins.