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产谷氨酸棒杆菌B9和T6—13的原生质体融合 被引量:2

Protoplast Fusion Of Corynebacterium B9 and T6 - 13 Strains of Producing Glutamic Acid
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摘要 棒杆菌B9和T6—13两菌株经UV变处理得到B9—2(SmR)和T6—13—3(RifR)两菌株,以此两菌株做为出发菌株。将对数前期的培养细胞经青霉素予处理及酶解制备原生质体,用40%PEG6000为助融剂,进行原生质体融合。用间接法检出具有Sm、Rif抗性的融合子。融合频率为6.55×10-6-1.64×10-5,融合子双抗性稳定,产谷氨酸,经摇瓶实验筛选出一株产谷氨酸明显高于亲本的融合子fu36。 Two mutant strains, B2(Smr)and T3(Rifr),Were obtained by UV mutagenesis of corynebacterinm b9 and t6-13.USing them as parent strains, the cultural Cells in early togarithmic phase was treated by penicillin firstly and then lysozyme, The protoplast were prepared.Protoplast fusion was made with the help of 40 % PEG6000, the fusants that had the resistance to sm and Rif were detectedby indirect method. The frequency of fusion was 6. 55 × 10-6-1. 64 × 10 × -5. Double resistance of the fusants is stable. By shake flat experiment, fusant fu36 was selected and amount of glutamic acid that produced were obriously higher than Parent strains.
出处 《生物技术》 CAS CSCD 1994年第1期40-44,共5页 Biotechnology
关键词 谷氨酸棒杆菌 原生质体融合 发酵 Protoplast fusion Corynebacterium glutamicum fusants.
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  • 1江行娟,杨庆云,任大明,冯德鑫,叶银凤,盛祖嘉.枯草杆菌中通过细胞融合的质粒转移[J]遗传学报,1981(01).

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