蔗糖酶和GOD的共固定化研究

Study about Co-immobilized Sucrase and GOD

本文研究了用吸附交联技术共固定化蔗糖酶和葡萄糖氧化酶（ＧＯＤ）的方法，考查了共固定化酶的动力学性质。试验结果表明：与溶液酶相比较，固定化蔗糖酶和ＧＯＤ的响应滞迟期分别为３分钟和２分钟，４态响应时间增加６分钟和４分钟，Ｋｍ值增大，ｐＨ─活力曲线变宽，最适ｐＨ值分别增大０．７和０．６４，最适温度则降低７．３℃和１６℃。以活性氧化铝作载体，戊二醛作交联剂制备的共固定化蔗糖酶和ＧＯＤ，其蛋白质固定化率为９２．９％，分解葡萄糖的总速度为４４１．６ＩＵ，当蔗糖浓度在０．２％以内时其反应速度与蔗糖浓度呈正相关（ｒ＝０．９９６），使用半衰期１６２３次，在４℃下保存１２０天活力残存为８３．７％。

It was researched that a method of co-immobilized sucrase and GOD by adsorption and crosslinkog,the kinetics.Compared with liquid enzymes, Km value of the sucrase or GOD was more large, the pH active curve more wide, arid the optimum pH value increased 0. 7 or 0. 64 separately, but the optimum temperture conversely decreased 7. 3℃ or 16 ℃.Besides the stead/ resPOnse thee was extended six or four mins, respectively,the hysteresis thee of response was equal to three or two mins.A ratio of the immobilized protein in co-immobilized granula that was prepared with active alumina supporter and cross-link agent of glutaric dialdehyde, was equal to 62. 9 percent, total reactive speed about oxidizing glucose 441. 6IU and the half-life 1623 times respectively and the retention activity 83. 7 percent after stored three mothes at temperatUre below 4℃. The reactive speed was proprtional to sucrase concentration which was at below 0. 2 percent.