碘乙酰胺和若丹明对植物原生质体存活率和植板车的影响

Effect of Iodoacetamide and Rodamine 6G on Plant Protoplast Viability and Plating Efficiency

本试验以普通小麦（Ｔａ）、多花黑麦草（Ｌｍ）和多年生黑麦草（Ｌｐ）细胞悬浮系来源的原生质体为材料研究了碘乙酰胺（ＩＯＡ）和若丹明（Ｒ—６Ｇ）对禾本科植物原生质休的失活效果。Ｔａ、Ｌｍ和ＬＰ原生质体经１ｍｍｏｌ／ＬＩＯＡ处理后存活率分另为３４％。１７％和０％，而对照的存活率分别为７８％、６８％和７１％。使Ｔａ和Ｌｍ原生质体全部失活的ＩＯＡ浓度需２ｍｍｏｌ／Ｌ。Ｔａ、Ｌｍ和Ｌｐ原生质体经４０μｇ／ｍｌＲ—６Ｇ处理后的存活率分别为６０％、６％和６３％，而对照的存活率分别为７０％、６１％和８７％。经４０μｇ／ｍｌＲ—６Ｇ处理后，Ｌｍ和ＬＰ原生质体植板率分别为０和１３细胞团／皿而对照的植板率分别为５９８和３９９细胞团／皿。以上结果表明，ＩＯＡ和Ｒ—６Ｇ都是有效的禾本科植物原生质体失活剂。

The metabolic inactivating effect of iodoacetamide (IOA) and Rodamine 6G (R-6G) on theprotoplasts isolated from the cell suspension cultures of Triticum aestivum L. (Ta), Lolium multiflorumLain. (Lin) and L. perenne L. (LP)was studied. The protoplast viability of Ta, Lm and Lp was34 %, 17% and 0% respectively when the protoplasts were treated with 1 mmol/L IOA, and was78 % , 68 % and 71 % respectively as control. The protoplasts of Ta and Lin were completely inactivated when IOA concentration reached 2 mmol/L. The protoplast viability of Ta, Lin and LP aws60%, 6 % and 63?A respectively, and the protoplast plating efficiency of Lin and LP was O and 13colonies per Petri dish when the protoplasts were treated with 40μg/ml R─6G; by comparison, theprotoplast viability of Ta, Lin and LP was 70%, 61 % and 87 % respectively, and the protoplast plating efficiency of Lin and LP was 598 and 399 colonies per petri dish in the control experiment. It wasthought that IOA and R-6G were effective in the protoplast inactivating treatment of gramineousspecies.