一种改进的杂交瘤细胞电融合方法

A MODIFIED ELECTROFUSION METHOD FOR THE GENERATION OF HYBRIDOMA CELLS

一种改进的杂交瘤细胞电融合方法邹翔（南京大学医学院南京２１０００８）刘琴芝，裴红英，郁文芳，安节，陈伯权（中国预防医学科学院病毒学研究所北京１０００５２）陈刚，赵南明（清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室北京１０００８４）我们对国...

In this paper we undertook to develop a modal electrofusion protocol, in which fusion of hybridoma Cells was induce by bipolar alternating pace, so as to further improve the efficiency of hybridoma production. Under optimal conditions, the exultant fusion frequency of murine hybridomas with the bipolar square wave pace was 2 - 3 fold greater than that with the unipolar pulses in both iso-osmotar and hypo-osmolar fuSion media. We suggest that the symmetrical pembilhation with bipolar alternating pace may amount for this strovement.