能化态与线粒体及其内膜体膜表面局部脱水

ENERGIZATION AND LOCAL DEHYDRATION OF MITOCHONDRIA AND MITOPLAST MEMBRANE SURFACE

荧光探针ＤＰＥ（Ｄｉｐａｌｍｉｔｏｙｌ－Ｎ－ＤａｎｓｙｌＰｈｏｓｐｈａｔｉｄｙｌｅｔｈａｎｏｌａｍｉｎｅ）标记于鼠肝线粒体及其内膜体膜表面，以测定其膜表面介电常数（ε）的变化与膜能化态的关系．在含有鱼藤酮的线粒体或其内膜体悬液中，加入琥珀酸，使膜处于能化态，可使膜表面。值分别下降１１％和２０％，分别再加ＫＣＮ，ＣＣＣＰ或Ｎｉｇｅｒｉｃｉｎ均能使ε值回升９％；若在上述悬液中，先加ＫＣＮ或ＣＣＣＰ，然后加入琥珀酸，则膜表面ε值无变化。若在线粒体或其内膜体悬液中，先加入ＣＣＣＰ或Ｎｉｇｅｒｉｃｉｎ，两者可使膜表面ε值升高１２％，其后加入鱼藤酮或琥珀酸等，则ε值均无变化，说明呼吸链抑制剂或解偶联剂抑制或解除膜的能化态，均可使膜表面ε值不发生变化。在另一组实验中，先加ＫＣＮ后，再加ＡＴｈ，使线粒体内膜处于能化态，表面ε值下降８％，此时再加寡霉素，则膜表面ε值相反上升１０％。这些实验事实均证明，线粒体膜处于能化态时，ε值下降，解除给化态，使ε值又上升。为能化态与线粒体膜表面水化力下降的相互关系提供了新信息．也为质子泵引起膜表面质子化，进而引起表面脱水，驱动膜融合的理论模型提供了新的证据。

A fluo probe, dansyl --p hos p hatidylethanolamine (DPE), was la belled o n the o uterne sauce of dschondria or boplast to show the changes in dielectric constant ofne sauce (b) with membrane ennyhation.Succinate was found to decrease e value ofthe rat liver chchondria or boplast by 11% or 20%. CCCP or ulgericin incths valueby 12%. Hoal, in the of KCN of CCCP, succinate was unable tO change s value. KCN, CCCP or nigericin could incme e value by about 9% if succinate was added atfnst. In the pace of KCN, hoplast sauce e value wu de by 8% additon ofAam, and incy by 10% by addition of oligomycin. All of the adults obtained shoalthat s value decthe with inelnbrane enwition and incpite acbderanedeenerghation. The Se are consideral as new infonnation concerning the ndtionship be energhahon and the dec in hydlahon of -chndria membrane surface,and also as new evidences for the model of proton pUInping-inducing cane fusion.