盐酸胍处理大麦类囊体膜对金属阳离子调节激发能分配的影响

EFFECT OF GUANIDINE HYDROCHLORIDE ON THE CATION-INDUCED EXCITATION ENERGY DISTRIBUTION IN THE BARLEY THYLAKID MEMBRANES

以大麦（ＨｏｒｄｅｕｍＶｕｌｇａｒｅＬ）叶片为实验材料，研究盐酸胍处理大麦类囊体膜对金属阳离子调节激发能分配的影响。结果表明：当未用盐酸胍处理时，Ｍｇ２＋对类囊体膜的Ｆ６８６／Ｆ７３６比值有显著的刺激作用，而用不同浓度盐酸胍处理类囊体膜，则明显阻滞Ｍｇ２＋调节激发能向ＰＳＩＩ分配，表明盐酸胍处理可能损伤类囊膜上ＬＨＣ—ＩＩ与Ｍｇ２＋调节作用有关的蛋白质，从而导致Ｍｇ２＋调节效应降低．用ＳＤＳ—ＰＡＧＥ方法研究盐酸胍处理后的类囊体膜色素蛋白复合物的组成变化，结果表明盐酸胍处理导致ＭＩＣ—ＩＩ中ＭＩＣＰ１和ＬＨＣＰ２组分消失，这说明盐酸胍处理阻滞Ｍｇ２＋调节效应可能与ＬＪＩＣ—ＩＩ上的ＬＨＣＰ１，ＬＨＣＰ２受损有关。

The results show that Mg2+ induce a sigifciant increase of Chla F686/F736 at 77 K of thylakoid membranes without GuHCl. With GuHCl thylakoid membranes treated the Mg2+ regulating excitation energy distribution effect was declined with the increase of the GuHCl concentration. The above evidence suppeds that GuHCl could damage proteins which is related to Mg2+-induced effects on LHC-II of thylakoid membranes. LHCP1 and LHCP2 components showed the disappearance of SDS-PAGE pattern of Chlorophyll-proteins complexes of the thylakoid memberanes treated with GuHCl. The results indicate that Mg2+-induced effects being lost on the thylkoid membranes modified by GuHCl may relate to the damage of LHCP1, LHCP2 components of LHC-II.