摘要
以大麦(HordeumVulgareL)叶片为实验材料,研究盐酸胍处理大麦类囊体膜对金属阳离子调节激发能分配的影响。结果表明:当未用盐酸胍处理时,Mg2+对类囊体膜的F686/F736比值有显著的刺激作用,而用不同浓度盐酸胍处理类囊体膜,则明显阻滞Mg2+调节激发能向PSII分配,表明盐酸胍处理可能损伤类囊膜上LHC—II与Mg2+调节作用有关的蛋白质,从而导致Mg2+调节效应降低.用SDS—PAGE方法研究盐酸胍处理后的类囊体膜色素蛋白复合物的组成变化,结果表明盐酸胍处理导致MIC—II中MICP1和LHCP2组分消失,这说明盐酸胍处理阻滞Mg2+调节效应可能与LJIC—II上的LHCP1,LHCP2受损有关。
The results show that Mg2+ induce a sigifciant increase of Chla F686/F736 at 77 K of thylakoid membranes without GuHCl. With GuHCl thylakoid membranes treated the Mg2+ regulating excitation energy distribution effect was declined with the increase of the GuHCl concentration. The above evidence suppeds that GuHCl could damage proteins which is related to Mg2+-induced effects on LHC-II of thylakoid membranes. LHCP1 and LHCP2 components showed the disappearance of SDS-PAGE pattern of Chlorophyll-proteins complexes of the thylakoid memberanes treated with GuHCl. The results indicate that Mg2+-induced effects being lost on the thylkoid membranes modified by GuHCl may relate to the damage of LHCP1, LHCP2 components of LHC-II.
出处
《生物物理学报》
CAS
CSCD
北大核心
1994年第2期187-192,共6页
Acta Biophysica Sinica
关键词
大麦
盐酸胍
类囊体膜
光合系统
金属
阳离子
Barley (Hodeum Vulgare L. ) Guanidine Hydrochloride (GuHCl)Thylakoid membranes Photosystem I Photospstem II