蛋白质酶法修饰的初步探讨──大豆蛋白和芝麻蛋白的合成类蛋白质研究

Preliminary Study on Enzymatic Modification of Protein──Study on Plastein Reaction of Soybean Protein with Sesame Protein

本文报道了以大豆蛋白和芝麻蛋白的胰蛋白酶水解物为原料，以胃蛋白酶为催化剂，通过合成类蛋白反应制取合成类蛋白质。结果表明，在上述反应中，胃蛋白酶是一种适宜的催化剂。底物水解度为８０％时效果最佳。大部分底物的分子量在１０００以下。底物最佳浓度为３０％，最佳ｐＨ为５．０。合成类蛋白质分子量约为１０，０００。

The plastein reaction by using tryptic hydrolyzate of soy protein and of sesame protein as substrates and pepsin as catalyst had been introduced. The results showed that the pepsin was a suitable catalyst for the plastein reaction of using tryptic hydrolyzate of soy protein and of sesame prorein. The best substrate was the hydrolyzate of the hydrolytic degree 80% ,and its molecular weight was below 1000 mostly, the optimum concentration of the substrate was 30% ;the optimum pH was 5. 0. The plastein synthesized had a molecular weight of synthesized plastein was about 10,000.