间期和有丝分裂期HeLa细胞中核仁蛋白B23的分布和含量的差异

DIFFERENCE IN THE DISTRIBUTION AND CONTENT OF NUCLEOLAR PROTEIN B 23 BETWEEN INTERPHASE AND MITOTIC HELA CELLS

B23蛋白是真核细胞核仁的两种主要蛋白成份之一。已往的工作表明,细胞内B23蛋白的分布与rRNA合成速率和细胞的生长状况密切相关。本工作利用抗B23蛋白单克隆抗体,研究了被两种作用于微管的药物秋水仙酰胺(colcemid)和紫杉酚(taxol)阻断的有丝分裂期和间期HeLa细胞内B23蛋白的分布和含量的差异。结果发现有丝分裂期细胞内B23蛋白含量明显高于间期细胞,而且B23蛋白在两类细胞中的分布也有明显的不同。

Protein B 23 is one of the two major protein components in eukaryocytic nuc-leolus. Previous studies indicate that the cellular content and distribution of protein B 23 are correlated with the synthetic rate of ribosomal RNA (rRNA) and cell growth. It is more abundant in tumor and growing cells than in normal resting cells. It may translocate from nucleolus to nuc-leoplasm after serum deprivation, or after being treated with RNA inhibitors such as actinomycin D, toyocamycin, or doxo-rubicin. In order to search for the role of protein B 23 in cell division, using an anti-B 23 monoclonal antibody, we detect the cellular content and distribution of protein B 23 in interphase and mitotic HeLa cells, respectively. Two microtubule drugs, col-cemid and taxol, are used to arrest the mitotic cells.The results show the cellular content of protein B 23 in mitotic cells is much higher than that in interphase cells. The intracellular distribution of protein B 23 in mitotic cells is distinguished from that in interphase cells too.