摘要
采用非树脂包进埋去包埋超薄切片电镜技术,并结合选择性生化抽提方法以及间接免疫荧光染色方法显示,在四膜虫(Tetrahymena thermophila)细胞内存在典型的核纤层结构。蛋白分离纯化与免疫印迹法分析结果说明四膜虫细胞核纤层可能主要由分子量为66KD的多肽构成。蛋白分离纯化结果表明四膜虫细胞的核纤层蛋白可能不如高等动物细胞的丰富。
The nuclear lamina in the nuclei of Tetrahymena thermophila was investigated using the techniques of selective extraction and embedment-free electron microscopy, as well as immunofluorescence staining. By means of immunoblotting and protein isolating, the lamina was found to consist mainly of a 66 KD polypeptide. While rabbit-anti-lamins polyclonal antibodies crossreacted with this protein, neither mu-rine-anti-lamin A&C mAB C 23 nor
murine-anti-lamin 1 mAB 223 showed positive reaction with it. It was also demonstrated that the lamin is not so rich as that in mammalian cells. The existence of lamina in T. thermophila, a unicellular organism, seems to show that the lamina is a basic characteristic structure of eu-karyotic cells, and that the function of the lamina may not be limited to the process of breakdown and reconstitution of nuclear envelope during mitosis.
出处
《实验生物学报》
CSCD
1994年第2期152-163,共12页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
关键词
四膜虫
核纤层
蛋白
鞭毛纲
Tetrahymena. Lamina. Lamin. Immunoblot.