人类抗中心体自身抗体的研究

STUDIES ON THE CENTROSOME ANTISERUM FROM A SCLERODERMA PATIENT

用间接免疫荧光法以小鼠腹水癌细胞为底物从硬皮病病人的自家抗血清中筛选出几个抗中心体的抗血清。因为中心体是化学构分复杂的细胞结构,而自家抗体又是多克隆的,故本文只用其中一个抗中心体抗血清作进一步研究。为了定位其抗原,同时也采用了L 929培养细胞为底物。发现这抗血清结合于微管,有丝分裂期的纺锤体,中心体以及其它一些核结构。同时也用此抗血清在细胞裂解液的免疫印迹膜上检出了分子量与微管蛋白相同的主要条带,此外还有几条较不明显的条带;后者肯定了间接免疫荧光的观察结果。

Using ascites cells as screening system and by means of indirect immunofluoresc-ence microscopy, several antisera against centrosome from scleroderma patients were discovered. Since centrosome is chemically complex cellular structure and the autoimmune antiserum is polyclonal, further investigation was made using one of the antisera against centrosome. L 929 cultured cells were also employed for the atnigen localisation. It was found that the antiserum decorated microtubules, mitotic spindle, centosome as well as some nuclear structure. Immunoblots of the cell lysate with the antiserum revealed that in addition to the main bands of tubulin there were several less distinct bands. This result confirmed the indirect immunofluores-cence observation.