幼年型类风湿性关节炎细胞免疫变化

Celluar Immunity in Patients with Juvenile Rheumatoid Arthritis (JRA)

研究目的探讨幼年型类风湿性关节炎（JRA）细胞免疫的改变及其临床意义。研究方法检测不同病期JRA患儿29例次，其中活动期14例；治疗后8例；缓解期7例。健康儿童19例为正常对照。采用间接免疫荧光法测定T淋巴细胞亚群，MTT比色法测定外周血单核细胞产生白细胞介素-2（IL－2）水平。研究结果活动期OKT3、OKT4、OKT4／OKT8和IL－2均明显降低；短期治疗后改变不明显；缓解期OKT4、OKT4／OKT8和IL－2明显升高，但仍较正常偏低。结论JRA患儿存在着细胞免疫异常，并且随着疾病向缓解期转化逐渐恢复，对临床诊断、治疗有指导意义。

Objective To evaluate the change of cellular immunity and its clinical significance in JRA.Methods 7 lymphocyte swbpopulation was analyzed by immunofluorescein and interleukin 2 (IL-2) produced by peripheral blood mononuclear cells in vitro by MTT colorimetric assay. 29 times of various stage with JRA were examined, including 14 clinically active patients, 8 posttreatment or 7 clinically inactive ones. There are 19 healthy children of similar age in control group.Results In active patients, the number of OKT8, OKT4, the ratio of OKT4/OKT8 and the level of IL-2 decreased significantly compared with normal controls. These changes recovered matkedly in remission patients though they did alter affective treatment for (2～4) weeks.Conclusion Patients with active JRA are characterised by aberration of cellular immunity and the aberration reverses obviously slow in comparision with the clinical manifestetions and the routine laboratory investigation.