摘要
研究目的探讨幼年型类风湿性关节炎(JRA)细胞免疫的改变及其临床意义。研究方法检测不同病期JRA患儿29例次,其中活动期14例;治疗后8例;缓解期7例。健康儿童19例为正常对照。采用间接免疫荧光法测定T淋巴细胞亚群,MTT比色法测定外周血单核细胞产生白细胞介素-2(IL-2)水平。研究结果活动期OKT3、OKT4、OKT4/OKT8和IL-2均明显降低;短期治疗后改变不明显;缓解期OKT4、OKT4/OKT8和IL-2明显升高,但仍较正常偏低。结论JRA患儿存在着细胞免疫异常,并且随着疾病向缓解期转化逐渐恢复,对临床诊断、治疗有指导意义。
Objective To evaluate the change of cellular immunity and its clinical significance in JRA.Methods 7 lymphocyte swbpopulation was analyzed by immunofluorescein and interleukin 2 (IL-2) produced by peripheral blood mononuclear cells in vitro by MTT colorimetric assay. 29 times of various stage with JRA were examined, including 14 clinically active patients, 8 posttreatment or 7 clinically inactive ones. There are 19 healthy children of similar age in control group.Results In active patients, the number of OKT8, OKT4, the ratio of OKT4/OKT8 and the level of IL-2 decreased significantly compared with normal controls. These changes recovered matkedly in remission patients though they did alter affective treatment for (2~4) weeks.Conclusion Patients with active JRA are characterised by aberration of cellular immunity and the aberration reverses obviously slow in comparision with the clinical manifestetions and the routine laboratory investigation.
出处
《实用儿科临床杂志》
CAS
CSCD
1994年第2期72-74,共3页
Journal of Applied Clinical Pediatrics
关键词
类风湿性
关节炎
细胞免疫
JRA
Cellular immunity
Interlukin-2
T-lymphocyte Subpopulation