Dot-IGSS,Dot-IGS和Dot-ELISA检测脑脊液中循环抗原诊断脑囊虫病的比较研究

DETECTION OF CIRCULATING ANTIGENS IN CEREBROSPINAL FLUID BY DOT-IMMUNOGOLD-SILVER STAINING (DOT-IGSS),DOT-IGS AND DOT-ELISA FOR DIAGNOSIS OF CEREBRAL CYSTICERCOSIS

应用自制的直径２０ｎｍ的胶体金标记ＧＡＲ－ＩｇＧ，以斑点免疫金银染色法（Ｄｏｔ－ＩＧＳＳ）、斑点免疫金染色法（Ｄｏｔ－ＩＧＳ）和Ｄｏｔ－ＥＬＩＳＡ三种方法，同时检测９９例脑囊虫病人脑脊液（ＣＳＦ）中循环抗原（ＣＡｇ），阳性率分别为９４．９５％、９３．９４％和９２．９３％。检测５１份非囊虫病患者和６２例其他脑部疾病患者的ＣＳＦ，三种方法均为阴性。诊断指数分别为１９４．９５％、１９３．９４％和１９２．９３％。检出ＣＡＳ的最小量Ｄｏｔ－ＩＧＳＳ为２４４ｎｇ／ｍｌ．Ｄｏｔ－ＩＧＳ和Ｄｏｔ－ＥＬＩＳＡ为４８８ｎｇ／ｍｌ。试验表明三种方法诊断脑囊虫病具有敏感性高、特异性强、重复性好和方法简便易行．有希望用于脑囊虫病的临床诊断。

GAR-IgG was labelled with 20 nm-diametered colloidal gold particles prepared in this laboratory. Detection of circulating antigens (CAg) of cerebrospinal fluid (CSF) by Dot-IGSS, DotImmunogold staining (Dot-IGS) and Dot-ELISA for diagnosis of 99 confirmed cases of cerebral cysticercosis. The positive rates of the Dot-IGSS, Dot-IGS and Dot-ELISA were 94. 95%,93. 94 % and 92. 93% respectively.Detection of CSF from 51 patients with non-cysticercosis and 62 patients with other cerebral diseases were all negative by these three methods. The detection indices were 194. 95%,193.94% and 192.93% respectively. The minimal CAg volume which could be detected was 244 ng/ml by Dot-IGSS and 488 ng/ml by Dot-IGS and Dot-ELISA.The results indicated the assays were highly specific, sensitive. reproducible and promising for the diagnosis of cerebral cysticercosis.