摘要
用人类乳头瘤病毒（ｈｕｍａｎｐａｐｉｌｌｏｍａｖｉｒｕｓ，ＨＰＶ）１６型ＤＮＡ，通过磷酸钙沉淀技术在体外成功地使ＮＩＨ３Ｔ３细胞发生恶性转化，转化细胞克隆后分析表明存在如下特征：转化细胞形态上变宽，呈多角状生长并形成集落；细胞的停泊依赖性下降；能在含１％小牛血清培养基中良好生长。转化细胞的密度依赖性下降。用Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交发现ＨＰＶ１６ＤＮＡ以整合状态存在于ＮＩＨ３Ｔ３细胞ＤＮＡ中。
Using calcium phosphate precipitation technique,we transformed in vitro NIH cell line successfully with human papilloma virus(HPV)type 16 DNA. Further analysis showed that the transformed cells possessed refractile,roundish,pile-up growth morphology,and decrease of anchorage dependence(growth in semi-fluid agar),serum dependence, and density dependence.Southern blot hybridization of transformed cell DNA indicated that HPV-16 DNA was incorporated into NIH 3T3 cells. The results indicate that HPV-16 DNA is responsible for the transformation of NIH 3T3 cells.
