慢性丙型肝炎患者外周血T淋巴细胞亚群的检测

Detection of Lymphocyte Subsets in Peripheral Blood of Patients with Hepatitis C

应用单克隆抗体和ＡＰＡＡＰ法对３５例慢性丙型肝炎患者的外周血Ｔ淋巴细胞亚群的分布进行了检测。结果表明慢性丙肝患者ＯＫＴ＿３￣＋细胞与正常对照无显著差异，而ＯＫＴ＿４￣＋、ＯＫＴ＿８￣＋细胞及ＯＫＴ＿４／ＯＫＴ＿８比值均低于正常对照。结果还表明ＨＢｓＡｇ阴性的丙肝患者细胞和ＯＫＴ＿４／ＯＫＴ＿８比值明显低于正常对照，而ＯＫＴ＿８￣＋细胞差别不显著。合并ＨＢｓＡｇ阳性的丙肝患者ＯＫＴ＿４￣＋、ＯＫＴ＿８￣＋细胞均显著低于正常对照和慢性活动性乙型肝炎患者，而ＯＫＴ＿４／ＯＫＴ＿８比值的差异则不显著，ＯＫＴ＿８￣＋细胞还显著低于ＨＢｓＡｇ阴性的丙肝患者。提示慢性丙型肝炎患者外周血Ｔ淋巴细胞亚群分布存在明显的失衡现象；合并ＨＢｓＡｇ阳性对ＯＫＴ＿８￣＋细胞有明显影响。

T cell subsets in peripheral blood of 35 chronic patients with hepatitis C have been investigated through monoclonal antibody and APAAP assay. The results showed that the percentage of cells in patient group with hepatitis C had no difference and cells and OKT4/OKT8 ratio were lower than that of the control group.The percentage of cells and OKT4/OKT8 ratio in HBsAg negative patient group with hepatitis C were significantly lower than that of the control group. No difference was observed in the percentage of OKT8 cells. The percentages of cells in HBsAg positive patient group with hepatitis C were significantly lower in comparison with control group and chronic active patient group with hepatitis B, but the OKT4/OKT8 ratio showed no difference. The percentage of cells was significantly lower as compared with HBsAg negative patient group with hepatitis C. These results indicated that T cell subsets in peripheral blood in patients with hepatitis C were obviously out of balance and the percentage of cells was significantly lower in hepatitis C patients co-infected with HBV.