整装培养细胞内质网的透射电镜观察

Electron Microscopic Observations of the Endoplasmic Reticulum in Whole-Mount Cultured Cells

采用以高锰酸钾为固定剂的整装培养细胞制样技术：观察了几种细胞系细胞（Ｈｅｌａ，ＬＡ_（７９５），２ＢＳ）内质网（ｅｎｄｏｐｌａｓｍｉｃｒｅｔｉｃｕｌｕｍ，简称ＥＲ）的超微结构。由ＥＲ小管构成的连续的网络系统存在于整个细胞的细胞质中。此法同样适于观察线粒体的超微结构及线粒体的三维空间关系，在Ｈｅｌａ细胞中，仅用高锰酸钾固定剂固定７ｍｉｎ就能清晰地看到线粒体的嵴。结果表明，本方法适于观察细胞中膜细胞器的分布和超微结构。

We have employed the technique of whole-mount cultured cell fixed with potassium permanganate to examine the ultrastructure of the endoplasmic reticulum(ER)in cells of different cell lines(Hela,LA(795)and 2BS).and revealed a continuous ER network consisting of interconnecting tubules extending throughout the cytoplasm.We have also examined the ultrastructure and threedimensional relationships of mitochondria.The cristae of mitochondria is clearly seen in Hela cells fixed with potassium permanganate for 7 min at room temperatrue. The results suggest that this method is very useful for direct examination of the ultrastructure and distribution of membrnae organelles.