银杏叶提取物对小鼠淋巴细胞和中性粒细胞活性的影响

Effects of the Extracts from the Leaves of Ginkgo Biloba on the Activity of Murine Lymphocytes and Neutrophils

用噻唑蓝［３－（４，５－ｄｉｍｅｔｈｙｌｔｈｉａｚｏｌ－２－ｙｌ）－２，５－ｄｉｐｈｅｎｙｌｔｅｔｒａｚｏｌｉｕｍｂｒｏｍｉｄｅ，ＭＴＴ］比色法和髓过氧化物酶（ｍｙｅｌｏｐｅｒｏｘｉｄａｓｅ，ＭＰＯ）微量测定法分别检测了银杏叶３种提取物对小鼠淋巴细胞活性和中性粒细胞吞噬杀伤功能的影响。结果显示，银杏叶提取物可明显增强小鼠淋巴细胞线粒体脱氢酶（Ｐ＜０．００１）和中性粒细胞ＭＰＯ的释放（Ｐ＜０．０１～０．００１）．由此可见，银杏叶提取物具有提高小鼠机体免疫细胞功能的作用。

The effects of extracts from the leaves of Ginkgo biloba on the activity of murine lymphocytes and neutrophils were investigated by colorimetric 3-(4,5-dimethylthiazol-2-y)-2, 5-diphenyltetrazolium bromide (MTT) assay and micromeasurement of myeloperoxidase (MPO). The results show that the activity of plastiosome dehydrogenase in lymphocytes (P<0. 001) and the MPO release of neutrophils(P<0. 01) were enhanced when the cells were pretreated with the extracts from the leaves of Ginkgo biloba. Together these findings indicate these extracts may strengthen the function of murine immunocytes.