摘要
应用制备电泳技术,从大阪鲫(CarassiusauratusCuvier)卵匀浆液中提纯两种卵黄蛋白—蛋白质H及蛋白质L。蛋白质H类似卵黄脂磷蛋白(磷为0.24%,氮为9.18%,等电点6.74),分子量471000。在SDS电泳中显示4条带,分子量分别为95800、90700、86500、26900。蛋白质L是类卵黄高磷蛋白,其分子量为94000。在SDS电泳中显示一条带(含磷8.85%、氰14.14%,等电点7.01),分子量29400。同时也观察到在不同pH值溶液中两种蛋白质紫外光吸收光谱和四阶导数谱变化的现象。
A preparative PAGE was used to purify two yolk proteins: protein H and protein L from homogenates of Carassius auratus Cuvier eggs. Protein H was a lipoprotein (phosphorus=0. 24%, nitrogen= 9. 18%, PI = 6. 74), similar to lipovitellin. Its mol. wt.was 471000, and displayed four bands in SDS-PAGE, corresponding to mol. wt. of 95800,90700, 86500, 26900 respectively. Protein L was a phosphoprotein (phosphorus = 8. 35% ,nitrogen= 14. 14%, PI= 7. 01 ) like phosvitin. But it had different amino acid composition.The mol. wt. of protein L was 94000, and displayed the band in SDS-PAGE,corresponding to mol. wt. of 29400. The change of ultraviolet absorption spectrum and four derivatives spectrum of these two proteins are observed in solutions of different PH values.
出处
《水产科技情报》
1994年第3期103-107,共5页
Fisheries Science & Technology Information
关键词
大阪鲫
卵巢
蛋白质
生化特性
提纯
Gonadal proteins
Lipovitellin
Molecular weight
Vitellogenin