人生长激素基因在团头鲂和鲤中的整合和表达

THE INTEGRATION AND EXPRESSION OF HUMAN GROWTH GENE IN BLUNT SNOUT BREAM AND COMMON CARP

采用显微注射方法，将带有小鼠ＭＴ－１基因启动顺序与人生长激素ｈＧＨ基因顺序重组的线状ＤＮＡ片段，注入团头鲂和鲤的受精卵，获得成活的实验鱼。经斑点、Ｓｏｕｔｈｅｒｎ杂交、Ｎｏｒｔｈｅｒｎ杂交、放射免疫和酶联等方法检测，表明外源基因在受体鱼中得到整合、转录、翻译和表达，并具促生长效应。转基因雌鱼和雄鱼有性繁殖所获得的子鱼带有外源基因，表明外源基因能通过性细胞传递给子代，并仍具促生长效应。

Linealized DNA fragments recombined out of the mouse gene promotor MT-1 and human growth hormone gene were microinjected into the fertilized eggs of blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio) respectively.By the inspecting methods of the dot blot, the Southern blot, the radioactive immunities and the enzymelinked immunosorbent assay, the integration, transcription, expression and growth promoting effect of the foreign genes in the receptive fish Were confirmed. The sexually-reproduced F, offsprings from transgenic male carps and female carps carry the foreign genes, thus showing that the foreign genes can be transmitted into F1 offsprings and have growth promoting effect.