摘要
采用显微注射方法,将带有小鼠MT-1基因启动顺序与人生长激素hGH基因顺序重组的线状DNA片段,注入团头鲂和鲤的受精卵,获得成活的实验鱼。经斑点、Southern杂交、Northern杂交、放射免疫和酶联等方法检测,表明外源基因在受体鱼中得到整合、转录、翻译和表达,并具促生长效应。转基因雌鱼和雄鱼有性繁殖所获得的子鱼带有外源基因,表明外源基因能通过性细胞传递给子代,并仍具促生长效应。
Linealized DNA fragments recombined out of the mouse gene promotor MT-1 and human growth hormone gene were microinjected into the fertilized eggs of blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio) respectively.By the inspecting methods of the dot blot, the Southern blot, the radioactive immunities and the enzymelinked immunosorbent assay, the integration, transcription, expression and growth promoting effect of the foreign genes in the receptive fish Were confirmed. The sexually-reproduced F, offsprings from transgenic male carps and female carps carry the foreign genes, thus showing that the foreign genes can be transmitted into F1 offsprings and have growth promoting effect.
出处
《水产学报》
CAS
CSCD
北大核心
1994年第4期284-289,共6页
Journal of Fisheries of China
关键词
人生长激素基因
整合
表达
团头鲂
鲤
human growth hormone gene
integration
expression
growth promoting effect
blunt snout bream
Megalobrama amblycephala
common carp
Cyprinus carpio